Immunogold colloidal gold—silver staining(1mmunogold-sliverstaining)Technical Department20century80ageHolgateA novel detection technique that combines immunogold staining and silver imaging methods. This technique is seen in light mirrors and also in electron microscopes. Often used in the recognition of proteins and nucleic acids after immunoblotting. The basic principle is to first perform immunogold staining to label the antigen, so that it binds to the location of the tissue cell antigen, and then add silver enhancement reagent, where silver ions are reduced in the reducing agent(hydroquinone)Under certain conditions, it is reduced to silver atoms, forming a "silver shell" on the gold particles. The "silver shell" itself also has a catalytic effect, which further promotes the reduction of more silver ions, making the "silver shell" larger and the antigen position clearer, and the antigen signal can be amplified. Therefore, this method is one of the most sensitive cellular chemical methods.

R-Gent SE-LMThe silver enhanced reagent kit can increase the average size of gold particles by depositing silver atoms, increase the visible radius of individual gold particles under a light microscope, increase the labeling density of small gold particles, and improve sensitivity.R-Gent SE-LMIt is a reagent kit specially designed for optical microscopes.

AURIONProduced by the companyR-Gent SE-LMThe reagent kit contains two types of reagents:developerandenhancerUsing a small reagent kit2*30mlFor example, the corresponding strengthened sample size can be estimated based on the amount of each sample used,100ul/Samples, the number of samples that can be made60One.1ml/In terms of samples, the number of detection samples that this small reagent kit can do is60One.

When operating, it is necessary to prepare in advancedeveloperandenhancerMixed,PHThe value is approximately8.3-8.5After adding the sample, this reagent is easy to apply and can be carried out under standard laboratory lighting conditions without avoiding light. Strengthening time requires exploration, typical15-30minute. exist40-60The phenomenon of self nucleation occurs within minutes.

characteristic:

l The silver enhancement reagent without photosensitivity provides further optical detection, and the black silver signal is not easily faded

l Can be used in conjunction with counterstain reagents under an optical microscope

l Detection in the field of biology has become simple and feasible

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